{"id":2556,"date":"2023-07-21T18:14:57","date_gmt":"2023-07-21T18:14:57","guid":{"rendered":"https:\/\/statorials.org\/my\/r-%e1%80%90%e1%80%bd%e1%80%84%e1%80%ba-quantile-normalization\/"},"modified":"2023-07-21T18:14:57","modified_gmt":"2023-07-21T18:14:57","slug":"r-%e1%80%90%e1%80%bd%e1%80%84%e1%80%ba-quantile-normalization","status":"publish","type":"post","link":"https:\/\/statorials.org\/my\/r-%e1%80%90%e1%80%bd%e1%80%84%e1%80%ba-quantile-normalization\/","title":{"rendered":"R \u1010\u103d\u1004\u103a quantile normalization \u101c\u102f\u1015\u103a\u1014\u100a\u103a\u1038"},"content":{"rendered":"<p><\/p>\n<hr>\n<p><span style=\"color: #000000;\">\u1000\u102d\u1014\u103a\u1038\u1002\u100f\u1014\u103a\u1038\u1005\u102c\u101b\u1004\u103a\u1038\u1007\u101a\u102c\u1038\u1019\u103b\u102c\u1038\u1010\u103d\u1004\u103a\u104a <a href=\"https:\/\/en.wikipedia.org\/wiki\/Quantile_normalization\" target=\"_blank\" rel=\"noopener\">\u1015\u1019\u102c\u100f\u1015\u102f\u1036\u1019\u103e\u1014\u103a\u1016\u103c\u1005\u103a\u1021\u1031\u102c\u1004\u103a\u1015\u103c\u102f\u101c\u102f\u1015\u103a\u1001\u103c\u1004\u103a\u1038<\/a> \u101e\u100a\u103a \u1000\u102d\u1014\u103a\u1038\u1002\u100f\u1014\u103a\u1038\u1006\u102d\u102f\u1004\u103a\u101b\u102c\u1002\u102f\u100f\u103a\u101e\u1010\u1039\u1010\u102d\u1019\u103b\u102c\u1038\u1014\u103e\u1004\u1037\u103a\u1015\u1010\u103a\u101e\u1000\u103a\u104d \u1016\u103c\u1014\u1037\u103a\u101d\u1031\u1019\u103e\u102f\u1014\u103e\u1005\u103a\u1001\u102f\u1000\u102d\u102f \u1011\u1015\u103a\u1010\u1030\u1016\u103c\u1005\u103a\u1005\u1031\u101e\u1031\u102c\u1014\u100a\u103a\u1038\u101c\u1019\u103a\u1038\u1010\u1005\u103a\u1001\u102f\u1016\u103c\u1005\u103a\u101e\u100a\u103a\u104b<\/span><\/p>\n<p> <span style=\"color: #000000;\">\u1021\u1031\u102c\u1000\u103a\u1016\u1031\u102c\u103a\u1015\u103c\u1015\u102b \u1025\u1015\u1019\u102c\u101e\u100a\u103a R \u1010\u103d\u1004\u103a \u1015\u1019\u102c\u100f\u1015\u102f\u1036\u1019\u103e\u1014\u103a\u1016\u103c\u1005\u103a\u1021\u1031\u102c\u1004\u103a\u101c\u102f\u1015\u103a\u1006\u1031\u102c\u1004\u103a\u1015\u102f\u1036\u1000\u102d\u102f \u1015\u103c\u101e\u1011\u102c\u1038\u101e\u100a\u103a\u104b<\/span><\/p>\n<h3> <span style=\"color: #000000;\"><strong>\u1025\u1015\u1019\u102c- R \u1010\u103d\u1004\u103a \u1015\u1019\u102c\u100f\u1015\u102f\u1036\u1019\u103e\u1014\u103a\u1016\u103c\u1005\u103a\u1001\u103c\u1004\u103a\u1038<\/strong><\/span><\/h3>\n<p> <span style=\"color: #000000;\">\u1000\u1031\u102c\u103a\u101c\u1036\u1014\u103e\u1005\u103a\u1001\u102f\u1015\u102b\u101b\u103e\u102d\u101e\u1031\u102c R \u1010\u103d\u1004\u103a \u1021\u1031\u102c\u1000\u103a\u1015\u102b\u1012\u1031\u1010\u102c\u1018\u1031\u102c\u1004\u103a\u1000\u102d\u102f \u1000\u103b\u103d\u1014\u103a\u102f\u1015\u103a\u1010\u102d\u102f\u1037\u1016\u1014\u103a\u1010\u102e\u1038\u101e\u100a\u103a\u1006\u102d\u102f\u1015\u102b\u1005\u102d\u102f\u1037\u104b<\/span><\/p>\n<pre style=\"background-color: #ececec; font-size: 15px;\"> <strong><span style=\"color: #008080;\">#make this example reproducible\n<\/span>set. <span style=\"color: #3366ff;\">seeds<\/span> (0)\n\n<span style=\"color: #008080;\">#create data frame with two columns\n<\/span>df &lt;- data. <span style=\"color: #3366ff;\">frame<\/span> (x=rnorm(1000),\n                 y=rnorm(1000))\n\n<span style=\"color: #008080;\">#view first six rows of data frame\n<\/span>head(df)\n\n           xy\n1 1.2629543 -0.28685156\n2 -0.3262334 1.84110689\n3 1.3297993 -0.15676431\n4 1.2724293 -1.38980264\n5 0.4146414 -1.47310399\n6 -1.5399500 -0.06951893<\/strong><\/pre>\n<p> <span style=\"color: #000000;\">x \u1014\u103e\u1004\u1037\u103a y \u1021\u1010\u103d\u1000\u103a quantile \u1019\u103b\u102c\u1038\u1000\u102d\u102f \u1010\u103d\u1000\u103a\u1001\u103b\u1000\u103a\u101b\u1014\u103a <a href=\"https:\/\/statorials.org\/my\/lapply-sapply-\u1014\u103e\u1004\u1037\u103a-tapply-r-\u1016\u103c\u1004\u1037\u103a-\u1021\u101e\u102f\u1036\u1038\u1001\u103b\u101b\u1014\u103a-\u101c\u1019\u103a\u1038\u100a\u103d\u103e\u1014\u103a\/\" target=\"_blank\" rel=\"noopener\">sapply()<\/a> \u1014\u103e\u1004\u1037\u103a <a href=\"https:\/\/statorials.org\/my\/r-\u1010\u103d\u1004\u103a-quantile-\u101c\u102f\u1015\u103a\u1006\u1031\u102c\u1004\u103a\u1001\u103b\u1000\u103a\/\" target=\"_blank\" rel=\"noopener\">quantile()<\/a> \u101c\u102f\u1015\u103a\u1006\u1031\u102c\u1004\u103a\u1001\u103b\u1000\u103a\u1019\u103b\u102c\u1038\u1000\u102d\u102f \u1021\u101e\u102f\u1036\u1038\u1015\u103c\u102f\u1014\u102d\u102f\u1004\u103a\u101e\u100a\u103a\u104b<\/span><\/p>\n<pre style=\"background-color: #ececec; font-size: 15px;\"> <strong><span style=\"color: #008080;\">#calculate quantiles for x and y\n<\/span>sapply(df, function(x) quantile(x, probs = seq(0, 1, 1\/4)))\n\n               xy\n0% -3.23638573 -3.04536393\n25% -0.70845589 -0.73331907\n50% -0.05887078 -0.03181533\n75% 0.68763873 0.71755969\n100% 3.26641452 3.03903341\n<\/strong><\/pre>\n<p> <span style=\"color: #000000;\">x \u1014\u103e\u1004\u1037\u103a y \u1010\u102d\u102f\u1037\u101e\u100a\u103a quantiles \u1021\u1010\u103d\u1000\u103a \u1021\u101c\u102c\u1038\u1010\u1030\u1010\u1014\u103a\u1016\u102d\u102f\u1038\u1019\u103b\u102c\u1038 \u101b\u103e\u102d\u101e\u1031\u102c\u103a\u101c\u100a\u103a\u1038 \u1010\u1030\u100a\u102e\u101e\u1031\u102c\u1010\u1014\u103a\u1016\u102d\u102f\u1038\u1019\u103b\u102c\u1038\u1019\u101f\u102f\u1010\u103a\u1000\u103c\u1031\u102c\u1004\u103a\u1038 \u101e\u1010\u102d\u1015\u103c\u102f\u1015\u102b\u104b<\/span><\/p>\n<p> <span style=\"color: #000000;\">\u1025\u1015\u1019\u102c\u104a x \u1021\u1010\u103d\u1000\u103a 25th \u101b\u102c\u1001\u102d\u102f\u1004\u103a\u1014\u103e\u102f\u1014\u103a\u1038\u1010\u1014\u103a\u1016\u102d\u102f\u1038\u101e\u100a\u103a <strong>-0.708<\/strong> \u1016\u103c\u1005\u103a\u1015\u103c\u102e\u1038 y \u1021\u1010\u103d\u1000\u103a 25th \u101b\u102c\u1001\u102d\u102f\u1004\u103a\u1014\u103e\u102f\u1014\u103a\u1038\u1010\u1014\u103a\u1016\u102d\u102f\u1038\u1019\u103e\u102c <strong>-0.7333<\/strong> \u1016\u103c\u1005\u103a\u101e\u100a\u103a\u104b<\/span><\/p>\n<p> <span style=\"color: #000000;\">\u1015\u1019\u102c\u100f\u1015\u102f\u1036\u1019\u103e\u1014\u103a\u1016\u103c\u1005\u103a\u1021\u1031\u102c\u1004\u103a\u101c\u102f\u1015\u103a\u1006\u1031\u102c\u1004\u103a\u101b\u1014\u103a R \u101b\u103e\u102d <a href=\"https:\/\/bioconductor.org\/packages\/release\/bioc\/html\/preprocessCore.html\" target=\"_blank\" rel=\"noopener\">preprocessCore<\/a> \u1021\u1011\u102f\u1015\u103a\u1019\u103e <strong>normalize.quantiles()<\/strong> \u101c\u102f\u1015\u103a\u1006\u1031\u102c\u1004\u103a\u1001\u103b\u1000\u103a\u1000\u102d\u102f \u1021\u101e\u102f\u1036\u1038\u1015\u103c\u102f\u1014\u102d\u102f\u1004\u103a\u1015\u102b\u101e\u100a\u103a\u104b<\/span><\/p>\n<pre style=\"background-color: #ececec; font-size: 15px;\"> <strong><span style=\"color: #008000;\">library<\/span> (preprocessCore)\n\n<span style=\"color: #008080;\">#perform quantile normalization\n<\/span>df_norm &lt;- as. <span style=\"color: #3366ff;\">data<\/span> . <span style=\"color: #3366ff;\">frame<\/span> ( <span style=\"color: #3366ff;\">normalize.quantiles<\/span> ( <span style=\"color: #3366ff;\">as.matrix<\/span> (df)))\n\n<span style=\"color: #008080;\">#rename data frame columns\n<\/span>names(df_norm) &lt;- c(' <span style=\"color: #ff0000;\">x<\/span> ', ' <span style=\"color: #ff0000;\">y<\/span> ')\n\n<span style=\"color: #008080;\">#view first six row of new data frame\n<\/span>head(df_norm)\n\n           xy\n1 1.2632137 -0.28520228\n2 -0.3469744 1.82440519\n3 1.3465807 -0.16471644\n4 1.2692599 -1.34472394\n5 0.4161133 -1.43717759\n6 -1.6269731 -0.07906793\n<\/strong><\/pre>\n<p> <span style=\"color: #000000;\">\u1011\u102d\u102f\u1037\u1014\u1031\u102c\u1000\u103a x \u1014\u103e\u1004\u1037\u103a y \u1021\u1010\u103d\u1000\u103a quantiles \u1019\u103b\u102c\u1038\u1000\u102d\u102f \u1011\u1015\u103a\u1019\u1036\u1010\u103d\u1000\u103a\u1001\u103b\u1000\u103a\u101b\u1014\u103a \u1021\u1031\u102c\u1000\u103a\u1015\u102b\u1000\u102f\u1012\u103a\u1000\u102d\u102f \u1021\u101e\u102f\u1036\u1038\u1015\u103c\u102f\u1014\u102d\u102f\u1004\u103a\u1015\u102b\u101e\u100a\u103a\u104b<\/span><\/p>\n<pre style=\"background-color: #ececec; font-size: 15px;\"> <strong><span style=\"color: #008080;\">#calculate quantiles for x and y\n<\/span>sapply(df_norm, function(x) quantile(x, probs = seq(0, 1, 1\/4)))\n\n               xy\n0% -3.14087483 -3.14087483\n25% -0.72088748 -0.72088748\n50% -0.04534305 -0.04534305\n75% 0.70259921 0.70259921\n100% 3.15272396 3.15272396\n<\/strong><\/pre>\n<p> <span style=\"color: #000000;\">\u101a\u1001\u102f \u1015\u1019\u102c\u100f\u1019\u103b\u102c\u1038\u101e\u100a\u103a x \u1014\u103e\u1004\u1037\u103a y \u1021\u1010\u103d\u1000\u103a \u1010\u1030\u100a\u102e\u1000\u103c\u1031\u102c\u1004\u103a\u1038 \u101e\u1010\u102d\u1015\u103c\u102f\u1015\u102b\u104b<\/span><\/p>\n<p> <span style=\"color: #000000;\">x \u1014\u103e\u1004\u1037\u103a y \u101e\u100a\u103a \u1015\u1019\u102c\u100f\u1015\u102f\u1036\u1019\u103e\u1014\u103a\u1016\u103c\u1005\u103a\u101e\u103d\u102c\u1038\u1015\u103c\u102e\u101f\u102f \u1000\u103b\u103d\u1014\u103a\u102f\u1015\u103a\u1010\u102d\u102f\u1037\u1015\u103c\u1031\u102c\u1014\u102d\u102f\u1004\u103a\u101e\u100a\u103a\u104b \u1010\u1005\u103a\u1014\u100a\u103a\u1038\u1006\u102d\u102f\u101b\u101e\u1031\u102c\u103a\u104a \u1016\u103c\u1014\u1037\u103a\u1016\u103c\u1030\u1038\u1019\u103e\u102f\u1014\u103e\u1005\u103a\u1001\u102f\u101e\u100a\u103a \u101a\u1001\u102f\u1021\u1001\u102b \u1000\u102d\u1014\u103a\u1038\u1002\u100f\u1014\u103a\u1038\u1002\u102f\u100f\u103a\u101e\u1010\u1039\u1010\u102d\u1019\u103b\u102c\u1038\u1014\u103e\u1004\u1037\u103a \u1010\u1030\u100a\u102e\u1015\u102b\u101e\u100a\u103a\u104b<\/span><\/p>\n<h3> <span style=\"color: #000000;\"><strong>\u1011\u1015\u103a\u101c\u1031\u102c\u1004\u103a\u1038\u1021\u101b\u1004\u103a\u1038\u1021\u1019\u103c\u1005\u103a\u1019\u103b\u102c\u1038<\/strong><\/span><\/h3>\n<p> <span style=\"color: #000000;\">\u1021\u1031\u102c\u1000\u103a\u1016\u1031\u102c\u103a\u1015\u103c\u1015\u102b \u101e\u1004\u103a\u1001\u1014\u103a\u1038\u1005\u102c\u1019\u103b\u102c\u1038\u101e\u100a\u103a R \u1010\u103d\u1004\u103a \u1021\u1001\u103c\u102c\u1038\u1018\u102f\u1036\u1021\u101c\u102f\u1015\u103a\u1019\u103b\u102c\u1038\u1000\u102d\u102f \u1019\u100a\u103a\u101e\u102d\u102f\u1037\u101c\u102f\u1015\u103a\u1006\u1031\u102c\u1004\u103a\u101b\u1019\u100a\u103a\u1000\u102d\u102f \u101b\u103e\u1004\u103a\u1038\u1015\u103c\u101e\u100a\u103a-<\/span><\/p>\n<p> <a href=\"https:\/\/statorials.org\/my\/r-\u1010\u103d\u1004\u103a\u1012\u1031\u1010\u102c\u1000\u102d\u102f\u1015\u102f\u1036\u1019\u103e\u1014\u103a\u1016\u103c\u1005\u103a\u1021\u1031\u102c\u1004\u103a\u1018\u101a\u103a\u101c\u102d\u102f\u101c\u102f\u1015\u103a\u1019\u101c\u1032\u104b\/\" target=\"_blank\" rel=\"noopener\">R \u1010\u103d\u1004\u103a\u1012\u1031\u1010\u102c\u1000\u102d\u102f\u1015\u102f\u1036\u1019\u103e\u1014\u103a\u1016\u103c\u1005\u103a\u1021\u1031\u102c\u1004\u103a\u101c\u102f\u1015\u103a\u1014\u100a\u103a\u1038<\/a><br \/> <a href=\"https:\/\/statorials.org\/my\/r-\u1010\u103d\u1004\u103a-\u101b\u102c\u1001\u102d\u102f\u1004\u103a\u1014\u103e\u102f\u1014\u103a\u1038\u1019\u103b\u102c\u1038\/\" target=\"_blank\" rel=\"noopener\">R \u1010\u103d\u1004\u103a \u101b\u102c\u1001\u102d\u102f\u1004\u103a\u1014\u103e\u102f\u1014\u103a\u1038\u1019\u103b\u102c\u1038 \u1010\u103d\u1000\u103a\u1014\u100a\u103a\u1038<\/a><br \/> <a href=\"https:\/\/statorials.org\/my\/r-\u1010\u103d\u1004\u103a-quantile-\u101c\u102f\u1015\u103a\u1006\u1031\u102c\u1004\u103a\u1001\u103b\u1000\u103a\/\" target=\"_blank\" rel=\"noopener\">R \u1010\u103d\u1004\u103a quantile() function \u1000\u102d\u102f\u1021\u101e\u102f\u1036\u1038\u1015\u103c\u102f\u1014\u100a\u103a\u1038<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u1000\u102d\u1014\u103a\u1038\u1002\u100f\u1014\u103a\u1038\u1005\u102c\u101b\u1004\u103a\u1038\u1007\u101a\u102c\u1038\u1019\u103b\u102c\u1038\u1010\u103d\u1004\u103a\u104a \u1015\u1019\u102c\u100f\u1015\u102f\u1036\u1019\u103e\u1014\u103a\u1016\u103c\u1005\u103a\u1021\u1031\u102c\u1004\u103a\u1015\u103c\u102f\u101c\u102f\u1015\u103a\u1001\u103c\u1004\u103a\u1038 \u101e\u100a\u103a \u1000\u102d\u1014\u103a\u1038\u1002\u100f\u1014\u103a\u1038\u1006\u102d\u102f\u1004\u103a\u101b\u102c\u1002\u102f\u100f\u103a\u101e\u1010\u1039\u1010\u102d\u1019\u103b\u102c\u1038\u1014\u103e\u1004\u1037\u103a\u1015\u1010\u103a\u101e\u1000\u103a\u104d \u1016\u103c\u1014\u1037\u103a\u101d\u1031\u1019\u103e\u102f\u1014\u103e\u1005\u103a\u1001\u102f\u1000\u102d\u102f \u1011\u1015\u103a\u1010\u1030\u1016\u103c\u1005\u103a\u1005\u1031\u101e\u1031\u102c\u1014\u100a\u103a\u1038\u101c\u1019\u103a\u1038\u1010\u1005\u103a\u1001\u102f\u1016\u103c\u1005\u103a\u101e\u100a\u103a\u104b \u1021\u1031\u102c\u1000\u103a\u1016\u1031\u102c\u103a\u1015\u103c\u1015\u102b \u1025\u1015\u1019\u102c\u101e\u100a\u103a R \u1010\u103d\u1004\u103a \u1015\u1019\u102c\u100f\u1015\u102f\u1036\u1019\u103e\u1014\u103a\u1016\u103c\u1005\u103a\u1021\u1031\u102c\u1004\u103a\u101c\u102f\u1015\u103a\u1006\u1031\u102c\u1004\u103a\u1015\u102f\u1036\u1000\u102d\u102f \u1015\u103c\u101e\u1011\u102c\u1038\u101e\u100a\u103a\u104b \u1025\u1015\u1019\u102c- R \u1010\u103d\u1004\u103a \u1015\u1019\u102c\u100f\u1015\u102f\u1036\u1019\u103e\u1014\u103a\u1016\u103c\u1005\u103a\u1001\u103c\u1004\u103a\u1038 \u1000\u1031\u102c\u103a\u101c\u1036\u1014\u103e\u1005\u103a\u1001\u102f\u1015\u102b\u101b\u103e\u102d\u101e\u1031\u102c R \u1010\u103d\u1004\u103a \u1021\u1031\u102c\u1000\u103a\u1015\u102b\u1012\u1031\u1010\u102c\u1018\u1031\u102c\u1004\u103a\u1000\u102d\u102f \u1000\u103b\u103d\u1014\u103a\u102f\u1015\u103a\u1010\u102d\u102f\u1037\u1016\u1014\u103a\u1010\u102e\u1038\u101e\u100a\u103a\u1006\u102d\u102f\u1015\u102b\u1005\u102d\u102f\u1037\u104b #make this example reproducible set. seeds (0) #create data frame with two columns df &lt;- data. frame (x=rnorm(1000), y=rnorm(1000)) #view first six rows of data frame head(df) xy 1 1.2629543 -0.28685156 2 -0.3262334 1.84110689 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